Analytical Chemistry with Laboratory

Lectures: 8 h 

Laboratory: 10 h

Main Topics:

Theoretical basis of chromatographic separations: thermodynamic aspects, kinetic theory of peak broadening and van Deemter’s equation. Chromatographic resolution equation. 
 
Gas chromatography: inlets, columns, instrumental parameters that affect the gas chromatographic separation.
 
Technical features in GC detectors. Iphenated mass spectrometry techniques with gas chromatography: electron ionization and chemical ionization techniques. Quadrupole analyzers.
 
Identification of a chromatographic peak: retention time and retention indexes. Quantitative analysis methods in chromatography: internal standard method, standard addition method, area normalization method.
 
Laboratory: analysis of a fragrance blend, identification and evaluation of its components.
 

Lecturers:
    Prof. Massimo Del Bubba, Dr. Lapo Renai
    Department of Chemistry “Ugo Schiff”
    University of Florence, Italy
    email: massimo.delbubba (at) unifi.it | lapo.renai (at) unifi.it 
    phone: + 39 055 4573326